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iscripttm reverse transcription supermix rt-qpcr kit  (Bio-Rad)


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    Structured Review

    Bio-Rad iscripttm reverse transcription supermix rt-qpcr kit
    Iscripttm Reverse Transcription Supermix Rt Qpcr Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscripttm+reverse+transcription+supermix+rt-qpcr+kit/iscript+reverse+transcription+supermix/pm38215866-90-24-26
    Average 90 stars, based on 1 article reviews
    iscripttm reverse transcription supermix rt-qpcr kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Retinoic acid fluctuation activates an uneven, direction-dependent network-wide robustness response in early embryogenesis
    Article Snippet: RNA samples were used for cDNA synthesis using the Bio-Rad iScriptTM Reverse Transcription Supermix for RT-qPCR kit (according to the manufacturer’s instructions).

    Article Title: FKF1 Interacts with CHUP1 and Regulates Chloroplast Movement in Arabidopsis.
    Article Snippet: First strand cDNA was synthesized using 0.5–1 μg DNA-free RNA with a iScriptTM reverse transcription supermix RT-qPCR kit (Bio-Rad, Hercules, CA, USA).

    Article Title: Processing of matched and mismatched rNMPs in DNA by archaeal ribonucleotide excision repair
    Article Snippet: Extracted RNA were quantified by Nanodrop 2000 (ThermoFisher) and 500 ng of RNA were used in Reverse Transcription reaction performed according to the manufacturer’s protocol (iScriptTM Reverse Transcription Supermix for RT-qPCR Kit, Bio-Rad).

    Expressing:

    Article Title: Non-targeted metabolomics analysis of cardiac Muscle Ring Finger-1 (MuRF1), MuRF2, and MuRF3 in vivo reveals novel and redundant metabolic changes
    Article Snippet: Total RNA was isolated by using TRIzol reagent according to the manufacturer's protocols (Ambion, Grand Island, NY). .. Briefly, 25 mg of ventricular tissue was homogenized in 1 ml TRIzol on ice (Fisher Scientific, Power Gen 125, setting 5). mRNA expression was determined using a two-step reaction: 1) cDNA was made using a iScriptTM Reverse Transcription Supermix for RT-qPCR kit (Cat.# 170-8841, BIO-RAD); 2) 2 μl cDNA was amplified on a Roche Lightcycler 480II system with exon-spanning Taqman Probes (Cat.# Mm01185221_m1, MuRF1/Trim63; Cat.# Mm01292963_g1, MuRF2/Trim55; Cat.# Mm00491308_m1, MuRF3/Trim 54; and Cat.# Hs99999901_s1, 18S, Applied Biosystems, Inc.) in 2× Lightcycler 480 Master Mix (Cat.# 04 707 494 001, Roche Diagnostics, Corp., Indianapolis, IN). ..

    Article Title: Influence of sex and liver cirrhosis on the expression of miR-146a-5p and its target genes, IRAK1 and TRAF6.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Reverse Transcription:

    Article Title: Non-targeted metabolomics analysis of cardiac Muscle Ring Finger-1 (MuRF1), MuRF2, and MuRF3 in vivo reveals novel and redundant metabolic changes
    Article Snippet: Total RNA was isolated by using TRIzol reagent according to the manufacturer's protocols (Ambion, Grand Island, NY). .. Briefly, 25 mg of ventricular tissue was homogenized in 1 ml TRIzol on ice (Fisher Scientific, Power Gen 125, setting 5). mRNA expression was determined using a two-step reaction: 1) cDNA was made using a iScriptTM Reverse Transcription Supermix for RT-qPCR kit (Cat.# 170-8841, BIO-RAD); 2) 2 μl cDNA was amplified on a Roche Lightcycler 480II system with exon-spanning Taqman Probes (Cat.# Mm01185221_m1, MuRF1/Trim63; Cat.# Mm01292963_g1, MuRF2/Trim55; Cat.# Mm00491308_m1, MuRF3/Trim 54; and Cat.# Hs99999901_s1, 18S, Applied Biosystems, Inc.) in 2× Lightcycler 480 Master Mix (Cat.# 04 707 494 001, Roche Diagnostics, Corp., Indianapolis, IN). ..

    Article Title: Influence of sex and liver cirrhosis on the expression of miR-146a-5p and its target genes, IRAK1 and TRAF6.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Quantitative RT-PCR:

    Article Title: Non-targeted metabolomics analysis of cardiac Muscle Ring Finger-1 (MuRF1), MuRF2, and MuRF3 in vivo reveals novel and redundant metabolic changes
    Article Snippet: Total RNA was isolated by using TRIzol reagent according to the manufacturer's protocols (Ambion, Grand Island, NY). .. Briefly, 25 mg of ventricular tissue was homogenized in 1 ml TRIzol on ice (Fisher Scientific, Power Gen 125, setting 5). mRNA expression was determined using a two-step reaction: 1) cDNA was made using a iScriptTM Reverse Transcription Supermix for RT-qPCR kit (Cat.# 170-8841, BIO-RAD); 2) 2 μl cDNA was amplified on a Roche Lightcycler 480II system with exon-spanning Taqman Probes (Cat.# Mm01185221_m1, MuRF1/Trim63; Cat.# Mm01292963_g1, MuRF2/Trim55; Cat.# Mm00491308_m1, MuRF3/Trim 54; and Cat.# Hs99999901_s1, 18S, Applied Biosystems, Inc.) in 2× Lightcycler 480 Master Mix (Cat.# 04 707 494 001, Roche Diagnostics, Corp., Indianapolis, IN). ..

    Article Title: Influence of sex and liver cirrhosis on the expression of miR-146a-5p and its target genes, IRAK1 and TRAF6.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Amplification:

    Article Title: Non-targeted metabolomics analysis of cardiac Muscle Ring Finger-1 (MuRF1), MuRF2, and MuRF3 in vivo reveals novel and redundant metabolic changes
    Article Snippet: Total RNA was isolated by using TRIzol reagent according to the manufacturer's protocols (Ambion, Grand Island, NY). .. Briefly, 25 mg of ventricular tissue was homogenized in 1 ml TRIzol on ice (Fisher Scientific, Power Gen 125, setting 5). mRNA expression was determined using a two-step reaction: 1) cDNA was made using a iScriptTM Reverse Transcription Supermix for RT-qPCR kit (Cat.# 170-8841, BIO-RAD); 2) 2 μl cDNA was amplified on a Roche Lightcycler 480II system with exon-spanning Taqman Probes (Cat.# Mm01185221_m1, MuRF1/Trim63; Cat.# Mm01292963_g1, MuRF2/Trim55; Cat.# Mm00491308_m1, MuRF3/Trim 54; and Cat.# Hs99999901_s1, 18S, Applied Biosystems, Inc.) in 2× Lightcycler 480 Master Mix (Cat.# 04 707 494 001, Roche Diagnostics, Corp., Indianapolis, IN). ..



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    Image Search Results


    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Development of VHL-recruiting STING PROTACs that suppress innate immunity

    doi: 10.1007/s00018-023-04796-7

    Figure Lengend Snippet:

    Article Snippet: iScriptTM Reverse Transcription Supermix for RT-qPCR , Bio-Rad , cat# 170-8891.

    Techniques: Transfection, Software, Reverse Transcription, SYBR Green Assay, Protease Inhibitor